Nov 20, 2008 | 10:51 pm

Rapeseed MS8, RF3 und MS8 x RF3


Trait: These rapeseed lines were developed to provide a pollination control system for the production of hybrid oilseed rape (MS8xRF3) expressing male sterility and tolerance to glufosinate-containing herbicides (BastaŽ, RelyŽ, FinaleŽ, and LibertyŽ).
Inserted genes in MS8
barnase-gene Source: Bacillus amyloliquefaciens

Function: Male sterility; the barnase gene encodes for a ribonuclease enzyme (RNAse). The RNAse interferes RNA production in specific cells of the pollen sac, thus disrupting their normal cell functioning and preventing the development of pollen.

bar-gene Source: Soil fungi (Streptomyces hygroscopius)

Function: The bar gene codes for the enzyme Phosphinothricin-Acetyltransferase (PAT) and leads to increased tolerance to glufosinate-containing herbicides.

Inserted genes in RF3
barstar-gene Source: Bacillus amyloliquefaciens

Function: Restoring fertility; transgenic RF3 plants contain the barstar gene to restore fertility in the hybrid line. The gene codes for a ribonuclease inhibitor (barstar), which specifically inhibits the barnase expressed by the MS8 line.

As a result, when pollen from the restorer line RF3 is crossed to the male sterile line MS8, the resultant progeny are able to develop normal anthers and pollen.

bar-gene Source: Soil fungi (Streptomyces hygroscopius)

Function: The bar gene codes for the enzyme Phosphinothricin-Acetyltransferase (PAT) and leads to increased tolerance to glufosinate-containing herbicides.

Methods for the genetic modification: Agrobacterium tumefaciens-mediated gene transfer.