Cotton MON15985 x MON1445

Trait: Resistance to lepidopteran pests and tolerance to glyphosate-containing herbicides (stacked hybrid produced by crossing lines MON15985 and MON1445).
Inserted gene:
cryIA(c)+cry2Ab2)-gene	Source:Bacillus thuringiensis kurstaki Function: These genes encode the Bt-toxins Cry1A(c) and Cry2Ab2, which confer resistance to lepidopteran pests of cotton, such as tobacco budworm (Heliothis virescens), cotton bollworm (Helicoverpa zea), pink bollworm (Pectinophora gossypiella), and soybean looper (Pseudoplusia includens).
cp4 epsps- gene	Source: Soil bacterium Agrobacterium ssp. strain CP4 Function: The cp4 epsps gene encodes for a version of EPSPS that is highly tolerant to inhibition by glyphosate and therefore leads to increased tolerance to glyphosate-containing herbicides.
Markergene	nptII (gene conferring resistance to kanamycin), gus-gene (reporter gene or visual marker gene)
Methods for the genetic modification: Microparticle bombardmentof plant cells tissue and Agrobacterium tumefaciens-mediated plant transformation. Event MON15985: The cry2Ab gene was introduced by microparticle bombardment into the cotton line DP50B already containing the cry1Ac gene. As a result, event 15985 expresses both the Cry1Ac and Cry2Ab insecticidal proteins. This event also contains the gus-gene from Escherichia coli. It was introduced as a visually scorable marker gene to identify transformed cells. Event MON 1445 expresses a naturally glyphosate tolerant version of the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (epsps) gene. The epsps gene was introduced by Agrobacterium tumefaciens-mediated plant transformation.