Feb 11, 2012 | 9:12 pm

Maize MON89034 x 1507 x MON88017 x 59122


Trait: The hybrid "SmartStax" MON89034 x 1507 x MON88017 x 59122 expresses novel insecticidal proteins: The Bt-toxins cry1A.105, cry2Ab2 and cryIF-gene, which confers resistance to lepidopterans as well as cry3Bb1-, Cry34Ab1 and Cry35Ab1-genes, which confers resistance to coleopterans.

The pat-gene and the cp4 epsps- gene conferring tolerance to  herbicides with the active ingredient glyphosate and glufosinate).

Inserted genes (MON89034):
cry1A.105
 and
cry2Ab2

Source: Bacillus thuringiensis 

Function: The toxins protects the plant from the lepidopteran insects, fall armyworm (Spodoptera sp.), black cutworm (Agrotis ipsilon), european corn borer(Ostrinia nubilalis) and the corn earworm (Helicoverpa zea).

Inserted genes (1507):
cryIF-gene

Source: Bacillus thuringiensis kurstaki

Function: The gene codes for a Bt-toxin, which protects the plant against the european corn borer (Ostrinia nubilalis) and other lepidopterans such as the pink borer (Sesamia spp.), fall armyworm (Spodoptera frugiperda), black cutworm (Agrotis ipsilon) and southwestern corn borer (Diatraea grandiosella).

pat-gene Source: Soil bacterium (Streptomyces viridochromogenes)

Function: This gene codes for the enzyme Phosphinothricin-Acetyltransferase (PAT) and leads to increased tolerance to glufosinate-containing herbicides.

This gene is also used as a marker gene.

Inserted genes (MON88017):
cry3Bb1-
genes

Source: Bacillus thuringiensis ssp. kumamotoensis

Function: The toxin protects the plant from the Coleopteran insects western corn rootworm (Diabrotica virgifera), northern corn rootworm (D. barberi), and Mexican corn rootworm (D. virgifera zeae).

cp4 epsps-
gene

Source: Soil bacterium Agrobacterium ssp. strain CP4

Function: The CP4 EPSPS gene encodes for a version of EPSPS that is highly tolerant to inhibition by glyphosate.

Inserted genes (59122):
Cry34Ab1,
Cry35Ab1-
genes

Source: Bacillus thuringiensis ssp. kumamotoensis

Function: The gene codes for a Bt-toxin, which protects the plant against Coleopteran insects, such as western corn rootworm (Diabrotica virgifera), northern corn rootworm (D. barberi), and mexican corn rootworm (D. virgifera zeae).

pat-gene
(marker
gene)
 

Function: This gene codes for the enzyme Phosphinothricin-Acetyltransferase (PAT) and leads to increased tolerance to glufosinate-containing herbicides.

Methods for the genetic modification: Agrobacterium tumefaciens-mediated gene transfer and Microparticle bombardment of plant cells tissue.