Sep 9, 2010 | 2:55 pm

Maize 3272


Trait: Thermostable alpha-amylase; for optimised bioethanol production.
Inserted genes:
amy797E-gene

Source: Thermococcales (thermostable bacterium)

Function: The gene encodes a thermostable alpha-amylase. This enzyme splits starch into smaller sugar subunits (saccharification). Splitting starch into sugar is the first step in producing bioethanol from plants.

Plants like maize naturally contain their own amylases. They get destroyed, however, when maize is subjected to high temperatures for ethanol production. Typically, supplemental amylase preparations must be added. Heat stable amalyse expressed in 3273 makes this step unneccessary.

Marker genepmi-gene

The pmi gene expresses the PMI protein, which allows the transformed plants to use mannose as an energy source.

Methods for the genetic modification: Agrobacterium tumefaciens-mediated gene transfer.